블라스팅과 양극산화된 티타늄 표면이 조골세포의 부착 및 분화에 미치는 영향
Effect of Blasted or Anodized Titanium Surface Roughness on Adhesion and Differentiation of Osteoblasts
박찬진, 조리라, 고성희, 엄흥식, 장범석,
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박찬진 ( Park Chan-Jin ) - 강릉대학교 치과대학 치과보철학교실
조리라 ( Cho Lee-Ra ) - 강릉대학교 치과대학 치과보철학교실
고성희 ( Ko Sung-Hee ) - 강릉대학교
엄흥식 ( Um Heung-Sik ) - 강릉대학교 치과대학 치주과학교실
장범석 ( Chang Beom-Seok ) - 강릉대학교 치과대학 치주과학교실
KMID : 0988720060220030261
Abstract
Effect of Blasted or Anodized Titanium Surface Roughness on Adhesion and Differentiation of Osteoblasts Chan-Jin Park*, Lee-Ra Cho*, Yang-Jin Yi*, Sung-Hee Ko** *Department of Prosthodontics, **Department of Pharmacology College of Dentistry, Kangnung National University and Oral Science Institute The success of an implant is determined by its integration into the tissue surrounding the biomaterial. Surface roughness is considered to influence the behavior of adherent cells. The aim of this in vitro study was to determine the effect of surface roughness on Saos-2 osteoblast-like cells. Titanium disks, blasted with 75 ㎛ aluminum oxide particles and anodic oxidized and machined titanium disks were prepared. Saos-2 were plated on the disks at a density of 50,000 cells per well in 48-well dishes. After 1 hour, 1 day, 6 days cell numbers were counted. One day, 6 days after plating, alkaline phosphatase(ALPase) activity was determined. Compared to experimental groups, the number of cells was significantly higher on control group. The stimulatory effect of surface roughness on ALPase was more pronounced on the experimental groups than on control group. These results demonstrate that surface roughness alters proliferation and differentiation of osteoblasts. The results also suggest that implant surface roughness may play a role in determining phenotypic expression of cells.
키워드
surface roughness;blasted;anodic oxidized;osteoblast-like cell
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